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Aerial line transect survey to estimate abundance of marsh deer (Blastocerus dichotomus) (Illiger, 1815) BABT
Andriolo,Artur; Piovezan,Ubiratan; Costa,Mateus José Rodrigues Paranhos da; Laake,Jeff; Duarte,José Maurício Barbanti.
The objective was to estimate abundance of marsh deer in the Paraná River basin of this work. The results provided information to support further analysis of the impact of the Porto Primavera flooding lake over population. Sixty-nine animals were recorded by aerial survey using distance sampling methodology. Animals were widely distributed throughout the study area. The uncorrected data resulted in a estimate density of 0.0035 ind/ha and a population size of 636 individuals. Correcting the g for the animals that could be missed the calculated abundance was 896 (CV=0.27) individuals. This methodology was applied with success to survey marsh deer. The result was important to evaluate the marsh deer status in the area, and for future analysis of the impact of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Abundance; Aerial Survey; Blastocerus dichotomus; Marsh deer; Distance Sampling.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000600017
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Aflatoxin M1 contamination in grated parmesan cheese marketed in Rio de Janeiro - Brazil BABT
Trombete,Felipe Machado; Castro,Izabela Miranda de; Teixeira,Alessandra da Silva; Saldanha,Tatiana; Fraga,Marcelo Elias.
This study aimed to determine the occurrence of AFM1 contamination in the samples of grated parmesan cheese marketed in the Metropolitan Region of Rio de Janeiro -Brazil. Thirty samples representing 10 major brands marketed in the region were analyzed by High Performance Liquid Chromatography with fluorescence detection (HPLC-FLD) after purification with immunoaffinity column. The method showed recovery values within the range of 70-90%, with RSD lower than 15% and limits of detection and quantification below the maximum level allowed by the European Commission for the presence of AFM1 in cheeses. The mycotoxin was identified in 18 (60%) of the grated cheese samples tested. The highest value corresponded to 0.69 ± 0.02 µg/kg and the mean for all the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycotoxin; Dairy products; HPLC-FLD.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200016
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Age and growth of Pimelodella pappenheimi (siluriformes, pimelodidae) from an Atlantic Forest Stream in Southern Brazil BABT
Amaral,Marina Faria do; Aranha,José Marcelo Rocha; Menezes,Márcia Santos de.
Studies were performed to determine the growth curves in length and weight for Pimelodella pappenheimi in the "das Pombas" stream, a coastal stream susceptible to the flash flood phenomenon. Samples were collected from September-95 to February-97, monthly, using electric fishing. The fishes were measured in total length and weight. A total of 156 pairs of otholits were taken for ring reading. The Ford-Walford transformation allowed the use of von Bertallanffy expression. The pale ring formation was annual, and it occurred between the summer and the autumn. This species grews in length according to Lt=15.86 (1-e-0.57(t+0.1551) ) equation and in weight according to Wt= 24.23 (1-e-0.57(t+0.1551) )3.12. The increase in length was faster until the second year...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Growth; Coastal Stream; Pimelodidae.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000400010
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Age-dependent fecundity of Podisus nigrispinus (Dallas) (Heteroptera: Pentatomidae) with sublethal doses of gammacyhalothrin BABT
Pereira,Alexandre Igor Azevedo; Ramalho,Francisco de Sousa; Bandeira,Catarina de Medeiros; Malaquias,José Bruno; Zanuncio,José Cola.
Podisus nigrispinus (Dallas) (Heteroptera: Pentatomidae) was exposed to gammacyhalothrin to study the stimulatory effect of low concentrations of this pyrethroid. The doses studied were 23.4375, 11.7188, 5.8594, 2.9297, 1.4648, 0.7324 mg.litre-1 and water, as control. The third, fourth, and fifth instars of P. nigrispinus were shorter with all the doses of this insecticide. The survival and longevity of this predator was highest with the lowest doses. The oviposition period of P. nigrispinus decreased as the doses increased. The lowest pre-and postoviposition periods were found with 11.7188 mg.litre-1 of gammacyhalothrin. The dose 0.7324 mg.litre-1 increased the clutch interval. The egg viability was similar between the treatments. The number of eggs per...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Predator; Pyrethroid; Stink bug; Hormesis; Biology.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500013
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Age-related Changes in DNA Methylation Status of hTERT Gene Promoter of Oral Epithelial Cells BABT
Bezerra,Stephane Flaviane de Oliveira; Costa,Ludimila de Araújo; Freitas,Priscylla Alves Nascimento de; Oliveira,Naila Francis Paulo de.
The purpose of this study was to investigate the effect of aging on the DNA methylation status of two genes involved in tumorigenesis (telomerase gene hTERT and DNA repair gene- MLH1) and one in metabolism (methylenetetrahydrofolate reductase gene- MTHFR) in oral epithelial cells. DNA methylation analysis was performed by Methylation Sensitive Restriction Enzymes (MSRE) of healthy oral epithelial cells of child (6-10 years, n=21), young (20-25 years, n=19) and elderly (over 60 years, n=25). The results for the hTERT gene showed significant variation in the methylation frequency at CpG dinucleotides among the groups (p=0.0001), with the methylated condition more frequently in children and young people. In relation to MLH1 and MTHFR, no differences were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Epigenetic; DNA methylation; Aging; DNA repair; Telomerase; MTHFR.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000100082
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Agrowaste-based Polyhydroxyalkanoate (PHA) production using hydrolytic potential of Bacillus thuringiensis IAM 12077 BABT
Gowda,Vaishnavi; Shivakumar,Srividya.
The study identified the innate enzymatic potential (amylase) of the PHB producing strain B.thuringiensis IAM 12077 and explored the same for cost-effective production of PHB using agrowastes, eliminating the need for pretreatment (acid hydrolysis and/or commercial enzyme). Comparative polyhydroxyalkanoate (PHA) production by B. thuringiensis IAM 12077 in biphasic growth conditions using glucose and starch showed appreciable levels of growth (5.7 and 6.8 g/L) and PHA production (58.5 and 41.5%) with a PHA yield of 3.3 and 2.8 g/L, respectively. Nitrogen deficiency supported maximum PHA yield (2.46 g/L) and accumulation (53.3%). Maximum growth (3.6 g/L), PHB yield (2.6 g/L) and PHA accumulation (72.8%) was obtained with C:N ratio of 8:1 using starch as the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus thuringiensis IAM 12077; Polyhydroxybutyrate; Nutrient limitation; Hydrolytic enzymes; Mango peel.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100009
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Alcoholic fermentation by the wild yeasts under thermal, osmotic and ethanol stress BABT
Silva,Rosimeire Oenning da; Batistote,Margareth; Cereda,Marney Pascoli.
This study aimed to explore the variability in the metabolism of nine wild yeasts isolated from the sugarcane juice from a distillery in the Brazilian State of Mato Grosso. Cell viability under the stress conditions was evaluated. The yeasts were inoculated in the test tubes containing sugarcane juice adjusted from 12 to 21º Brix, ethanol from 6 to 12% in volume and temperature at 30, 35 and 40ºC. The viability was established by the growth in Petri dishes and visually by the CO2 production in the test tubes. None of the evaluated yeasts showed simultaneous resistance to the three stress conditions. The potential of yeast BB.09 could be emphasized due to its ability to ferment up to12% ethanol at 30°C.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bio prospection; Alcohol distillery; Cellular viability; Fermentation.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000200001
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Alkaline gel electrophoresis assay to detect DNA strand breaks and repair mechanisms in Escherichia coli BABT
Mattos,José Carlos Pelielo de; Motta,Ellen Serri da; Oliveira,Márcia Betania Nunes de; Dantas,Flávio José da Silva; Araujo,Adriano Caldeira de.
Reactive oxygen species (ROS) can induce lesions in different cellular targets, including DNA. Stannous chloride (SnCl2) is a ROS generator, leading to lethality in Escherichia coli (E. coli), with the base excision repair (BER) mechanism playing a role in this process. Many techniques have been developed to detect genotoxicity, as comet assay, in eukaryotic cells, and plasmid DNA agarose gel electrophoresis. In this study, an adaptation of the alkaline gel electrophoresis method was carried out to ascertain the induction of strand breaks by SnCl2 in bacterial DNA, from E. coli BER mutants, and its repair pathway. Results obtained show that SnCl2 was able to induce DNA strand breaks in all strains tested. Moreover, endonuclease IV and exonuclease III play...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alkaline gel electrophoresis; Escherichia coli; Base excision repair; Stannous chloride; DNA strand breaks; Reactive oxygen species.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700020
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Alkaline phosphatase activity in plasma and liver of rats submitted to chronic exposure to fluoride BABT
Fernandes,Mileni da Silva; Iano,Flávia Godoy; Rocia,Vivian; Yanai,Marcela Mitsuko; Leite,Aline de Lima; Furlani,Tatiana Almeida; Buzalaf,Marília Afonso Rabelo; Oliveira,Rodrigo Cardoso de.
The aim of this study was to compare the effect of fluoride (F) on alkaline phosphatase activity in the liver and plasma of the rats. Four groups of male Wistar rats (n=6), which received drinking water containing 5, 15 or 50 ppm F or deionized water (control) throughout the experiment were included in the study. The animals were euthanized and had their tissues and blood plasma collected for the analysis of fluoride and alkaline phosphatase. There was an increase in F concentration in most tissues in the animals treated with higher F concentrations, except for the heart. The alkaline phosphatase assay showed an increase in the activity in the liver and blood plasma of the animals treated with fluoride concentrations of 15 and 50 ppm (p<0.05). This...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alkaline phosphatase; Fluoride; Liver; Kidney.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600014
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Alkaloids and triterpene from Almeidea coerulea (Nees and Mart.) a. St.-Hil. and anti-leishmanial activity BABT
Cortez,Lucia Elaine Ranieri; Ferreira,Izabel Cristina Piloto; Lonardoni,Valdrinez Campana; Ferreira,Antonio Gilberto; Vieira,Paulo Cesar; Silva,Maria Fátima das Graças Fernandes da; Fernandes,João Batista; Cortez,Diógenes Aparicio Garcia.
The dichloromethane extract of Almeidea coerulea stems yielded the (11-hydroxyrutaecarpine alkaloid reported for the first time from this species) and the triterpene (28-hydroxy-28, 29-dihydrolupeol). The dictamine, skimianine, sitosterol and stigmasterol were also isolated from methanol extract. Extracellular forms of Leishmania amazonensis (promastigotes) was tested with dichloromethane extract and 28-hydroxy-28, 29-dihydrolupeol with showed anti-leishmanial activity above 0.1 mg/mL and 75µg/mL (inhibited 50% promastigote growth), respectively.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Almeidea coerulea; Rutaceae; Indolopyridoquinazoline alkaloid; Anti-leishmanial activity.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000100008
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Allelopathic evidence in Brachiaria decumbens and its potential to invade the Brazilian Cerrados BABT
Barbosa,Elizabeth Gorgone; Pivello,Vânia Regina; Meirelles,Sérgio Tadeu.
The aim of this study was to look for evidence of allelochemicals in B. decumbens, in parts of the plant from where they could easily be released to the environment. The germination inhibition of Phalaris canariensis, Lactuca sativa (standard species) and Melinis minutiflora, another invasive African grass, was tested using B. decumbens germinating seeds and aqueous leachates of the roots, green and senescent leaves, at 5, 10 and 20% w/v. Both the germinating seeds and the aqueous leachates of B. decumbens reduced the germination of the species tested; the effectiveness of the aqueous leachates increased according to concentration. Apparently, the competitive advantage of B. decumbens in the cerrados could be amplified via allelopathy.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Allelopathy; Cerrado; Biological invasion; Brachiaria decumbens; Melinis minutiflora.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400021
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Allelopathic evidence in exotic guava (Psidium guajava L.) BABT
Chapla,Tatiani Elisa; Campos,João Batista.
The allelopathic activity of the leaf aqueous extract of guava (Psidium guajava L.) on seed germination and root growth in lettuce (Lactuca sativa L.) was studied in an experiment consisting of four replicates with 10 seeds each and extract concentrations of 5, 10, and 20%. Guava allelopathic activity occurred during the germination and growth of lettuce roots at 20% extract concentration, as shown by ANOVA. This result suggested the allelopathic potential of the species. Therefore, the success of P. guajava in invading environments could be partially due to allelopathy. Further studies using other plant parts and field experiments should be conducted.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Abandoned pastures; Allelopathy; Biological invasion; Riparian vegetation; Seeds.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600012
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Allelopathic potential of orsellinic acid derivatives BABT
Peres,Marize Terezinha Lopes Pereira; Mapeli,Ana Maria; Faccenda,Odival; Gomes,Alcir Teixeira; Honda,Neli Kika.
Environmental degradation caused by the excessive use of pesticides has motivated studies aimed at the discovery of new formulations for pest control. The bioassays carried out under laboratory conditions for the present study involved the germination and seedling growth of Lactuca sativa cv. Grand Rapids and Allium cepa cv. Baia Periforme treated with lecanoric acid or one of seven esters obtained from the alcoholysis of the acid. Each substance was tested at concentrations of 10-3, 10-5, 10-7 M, and 10-9 M. The results revealed that, except for lecanoric acid and methyl orsellinate, all other compounds exhibited significant differences in their action when compared with the controls. Also, A. cepa was more sensitive to the compounds assayed, at the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Allelopathy; Germination; Growth; Lactuca sativa; Allium cepa; Orsellinates.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400027
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Alleviating soil acidity through plant organic compounds BABT
Meda,Anderson R.; Cassiolato,Marcelo E.; Pavan,Marcos A.; Miyazawa,Mário.
A laboratory experiment was conducted to evaluate the effects of water soluble plant extracts on soil acidity. The plant materials were: black oat, oil seed radish, white and blue lupin, gray and dwarf mucuna, Crotalaria spectabilis and C. breviflora, millet, pigeon pea, star grass, mato grosso grass, coffee leaves, sugar cane leaves, rice straw, and wheat straw. Plant extracts were added on soil surface in a PVC soil column at a rate of 1.0 ml min-1. Both soil and drainage water were analyzed for pH, Ca, Al, and K. Plant extracts applied on the soil surface increased soil pH, exchangeable Ca ex and Kex and decreased Al ex. Oil seed radish, black oat, and blue lupin were the best and millet the worst materials to alleviate soil acidity. Oil seed radish...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Plant residue; Organic material; Leaching.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000200012
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Allochthonous matter input in two different stretches of a headstream (Itatinga, São Paulo, Brazil) BABT
Afonso,Ana Adelina de O.; Henry,Raoul; Rodella,Rita C.S.Maimoni.
In two streches of the Itaúna stream, one in a gallery forest and the other in an herbaceous vegetation zone, litter matter input (sorted by leaves, ligneous and reproductive components) were measured during a year. In the gallery forest stretch, the direct input and lateral transport attained 713 g.m-2.year-1 and 421 g.m-1. year-1, respectively. In contrast, the herbaceous vegetation stretch showed a direct litter input and lateral transport of only 86 g.m-2.year-1 and 124 g.m-1. year-1, respectively. The empoverishment of riparian vegetation had a significant effect on the decrease of allochthonous input to stream mainly by direct input. Peaks of direct input into Itaúna stream occurred during late dry season and in the early wet season. They were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Allochthonous input; Stream; Riparian vegetation.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000300014
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Alpha amylase from a fungal culture grown on oil cakes and its properties BABT
Ramachandran,Sumitra; Patel,Anil K.; Nampoothiri,Kesavan Madhavan; Chandran,Sandhya; Szakacs,George; Soccol,Carlos Ricardo; Pandey,Ashok.
Solid-state fermentation was carried out for the production of alpha-amylase using Aspergillus oryzae. Different oil cakes such as coconut oil cake (COC) sesame oil cake (SOC), groundnut oil cake (GOC), palm kernel cake (PKC) and olive oil cake (OOC) were screened to be used as substrate for the enzyme production and also compared with wheat bran (WB). GOC was found to be the best producer of the enzyme among these. Combination of WB and GOC (1:1) resulted higher enzyme titres than the individual substrates. Maximum amount of enzyme (9196 U/gds) was obtained when SSF was carried out using WB + GOC, having initial moisture of 64% and supplemented with lactose and ammonium nitrate (1% each) at 30ºC for 72h using 2 mL spore suspension (6x10(7)spores/ml)....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alpha amylase; Oil cakes; Solid-state fermentation; Partial purification; Aspergillus oryzae.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000200019
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Alterations in Biomarkers Associated with Sterility in Pectinophora gossypiella (Saunders) Induced by Gamma Irradiation BABT
Ali,Hanaa Mahmoud; Moustafa,Hemat Zakaria; Sayed,Rehab Mahmoud.
ABSTRACT Nowadays, radiation technology is widely used to produce changes in Biosystems. The goal of this work is to determine the variation induced male Pectinophora gossypiella in gamma-irradiated as pupae using 50Gy and 150Gy. Comparing elements composition and DNA (using RAPD-PCR) between substerile 50Gy and the sterile dose 150Gy in P. gossypiella showed variation between them. Potassium (K) was the most abundant elements in unirradiated and irradiated males followed by magnesium (Mg). The percentage of heavy metals as copper, zinc, and cadmium concurrent with K was directly proportional to the radiation dose. While the percentage of Mg, Phosphorous and calcium decreased as the radiation dose increased. The results also revealed that some extra bands...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pectinophora gossypiella; Gamma rays; RAPD-PCR; Elements; DNA.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100401
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Alterations in Biomarkers Associated with Sterility in Pectinophora gossypiella (Saunders) Induced by Gamma Irradiation BABT
Ali,Hanaa Mahmoud; Moustafa,Hemat Zakaria; Sayed,Rehab Mahmoud.
ABSTRACT Nowadays, radiation technology is widely used to produce changes in Biosystems. The goal of this work is to determine the variation induced male Pectinophora gossypiella in gamma-irradiated as pupae using 50Gy and 150Gy. Comparing elements composition and DNA (using RAPD-PCR) between substerile 50Gy and the sterile dose 150Gy in P. gossypiella showed variation between them. Potassium (K) was the most abundant elements in unirradiated and irradiated males followed by magnesium (Mg). The percentage of heavy metals as copper, zinc, and cadmium concurrent with K was directly proportional to the radiation dose. While the percentage of Mg, Phosphorous and calcium decreased as the radiation dose increased. The results also revealed that some extra bands...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pectinophora gossypiella; Gamma rays; RAPD-PCR; Elements; DNA.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100436
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Alterations in proteins and amino acids of the Nile cyanobacteria Pseudanabaena limnetica and Anabaena wisconsinense in response to industrial wastewater pollution BABT
El-Sheekh,Mostafa Mohamed; El-Otify,Ahmed Mohamed; Saber,Hani.
The effect of industrial wastewater on the Nile cyanobacteria Pseudanabaena limnetica and Anabaena wisconsinense was investigated. The data showed that P. limnetica was more sensitive to pollution than A. wisconsinense. The treatments with different levels of wastewater exerted pronounced reductions in protein and amino acids content. SDS-PAGE analysis revealed that the cyanobacteria grown in the industrial wastewater showed induction in the synthesis of certain polypeptides and repression of others. The treatments of P. limnetica with wastewater stimulated the appearance of six protein bands with molecular masses of 28, 30, 31, 32, 58 and 97 kDa. The same treatments caused the disappearance of 20, 38 and 56 kDa. The structural protein pattern of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amino acids; Anabaena; Electrophoretic patterns; Nile cyanobacteria; Protein; Pseudanabaena; Wastewater.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400022
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Alterations on growth and gill morphology of Danio rerio (Pisces, Ciprinidae) exposed to the toxic sediments BABT
Fracácio,Renata; Verani,Nelsy Fenerich; Espíndola,Evaldo Luiz Gaeta; Rocha,Odete; Rigolin-Sá,Odila; Andrade,Cássio Arilson.
The objective of this work was to assess the toxicity of sediment samples from six cascade reservoirs in Tietê river system, in São Paulo State, Brazil, through chronic-partial toxicity bioassays with Danio rerio larvae as test-organisms. Histology of gills and biometric measurements were used to determine the existence of toxicity problems. The alterations on gill morphology here detected were considered of first stage (hyperplasia, lamellar junction and excess of mucous cells) in the first reservoirs and very slight in the last ones. The biometric analysis pointed to inadequate conditions for the growth of the test-organisms when exposed to the sediment of the rivers and upstream reservoirs and also indicated an improvement of environmental conditions...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gill morphology; Sediment toxicity; Danio rerio; Tietê reservoirs; Pollution.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000400023
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